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Metal tolerance may have evolved on either naturally metal-enriched soils or on anthropogenic sites that have been generated by waste deposits from ore mining or metal smelter activity (Ernst et al. In contrast to natural metalliferous soils, industrial sites are younger and generally show higher metal concentrations.These differences in site history may affect the evolutionary processes and selection pressure dynamics.
The majority of calamine species are native and widespread in the area surrounding the heaps (Jędrzejczyk-Korycińska ).Furthermore, one occurrence on a non-metalliferous lowland site in Zagórzyce (Przemyski and Piwowarczyk ; subsequently referred to as PL10) has recently been mentioned.Populations from both the lowland waste heap sites and the Tatra Mts have been identified as diploids (2n = 18) in a caryological study (Skalińska ; Babst-Kostecka et al. We surveyed the nuclear genome diversity to examine population-level microevolutionary processes in the context of environmental and geographic variation.Soil p H was measured in distilled water by means of the potentiometric method (Wąchalewski The collected leaf material was dried for 24 h at 55 °C prior to DNA extraction from 10 to 15 mg of dry material using the Nucleo Spin® 8/96 Plant kit (Macherey-Nagel®). All individuals were genotyped using 9 microsatellite markers developed in .Several loci were amplified simultaneously and using forward primers labelled with Applied Biosystems dyes (multiplex 1: B6, B66, B45, B102, B146 and multiplex 2: B3, B24, B8, B26).Additional factors that could influence the overall level of genetic diversity should be considered, including i) the age of polluted sites, ii) the level of soil metal concentration and associated selective pressure, iii) the level of genetic connectivity between M and NM populations, iv) the possibility of successive colonization events from adjacent NM populations, and v) some species-specific features such as clonal growth, sensitivity to abiotic stress, and constitutive (i.e.
species wide) or population-specific metal tolerance (Bickham et al. So far it has remained largely unclear whether historical, ecological or biogeographic factors could influence the pattern of genetic structure among M and NM populations of pseudometallophytes (Ye et al. Mining and processing of rich zinc-lead (Zn-Pb) ore in the Bolesław - Olkusz region (Cracow - Silesian Upland) dating back to the 13th century has created some of the largest and most polluted anthropogenic metalliferous sites in Europe, with waste heaps and dust deposits of different age, composition and metal concentrations (Cabala et al.
These clear divergence patterns promote The adaptation of organisms to natural and/or anthropogenic changes in their physical environment has raised considerable interest among plant ecologists and evolutionary biologists.
Special attention has been given to living organisms occurring on sites with high concentrations of Metal Trace Elements (hereafter called “metals”).
Populations clustered into two groups which corresponded to their edaphic origin and diverged 1,200 generations ago.
We detected a significant decrease in genetic diversity and evidence for a recent bottleneck in metallicolous populations.
Pseudometallophytes are model organisms for adaptation and population differentiation because they persist in contrasting edaphic conditions of metalliferous and non-metalliferous habitats.